Fillable Printable Project Research Roadmap Template
Fillable Printable Project Research Roadmap Template
Project Research Roadmap Template
SAMPLE
Research Project Research Roadmap/Associated
Budget
TITLE: Development of a Bio-mimetic Drug Delivery System
FUNDING AGENCIES: Goldman Philanthropic Partnerships, Rockefeller Brothers
Fund
GPP PROJECT CODE #: 03-0331IK
FUNDING PERIOD: April 1, 2003-March 31-2004
TOTAL OPERATING BUDGET: $25,000
SPECIFIC AIMS AND OBJECTIVES OF RESEARCH PLAN
First Quarter: April 1, 2003-June 30, 2003
Research Objective 1: Expression/Purification of proteins P1, P2
Research Objective 2: Synthesis of lipid analogs to P1, P2
Second Quarter: July 1, 2003-Sept. 30, 2003
Research Objective 3: Preparation and characterization of liposomes
Research Objective 4: Incorporation of molecules into liposomes
Third Quarter: October 1, 2003-December 31, 2003
Research Objective 5: Release studies of model components using fluorescent-labeled
tracers
Research Objective 6: Binding studies on fixed cells
Fourth Quarter: January 1, 2004-March 31, 2004
Research Objective 7: In vitro cell culture binding studies
Research Objective 8: Uptake and endosomal drug release evaluation
SAMPLE
TITLE:
FUNDING AGENCIES: Goldman Philanthropic Partnerships, Rockefeller Brothers
Fund
FUNDING PERIOD: April 1, 2003-June 30, 2003
RESEARCH PLAN BUDGET
A. Budget for Research Plan, 1st quarter:
1. Salary and Fringe Benefits
Principal Investigator: Ilya Koltover, PhD .................................. $0.00
Project Investigator: Jianqin Liu, PhD (Time – 25%) ................. $2473.00
2. Materials, Reagents and Supplies................................................ $3000.00
3. Analytical Service ........................................................................ $314.00
4. Indirect Expenses......................................................................... $463.00
Total First Quarter Research Budget ...................................................... $6250.00
TITLE:
FUNDING AGENCIES: Goldman Philanthropic Partnerships
FUNDING PERIOD: July 1, 2003-Sept. 30, 2003
RESEARCH PLAN BUDGET
A. Budget for Research Plan, 2nd quarter:
1. Salary and Fringe Benefits
Principal Investigators: Ilya Koltover, PhD................................. $0.00
Project Investigator: Jianqin Liu, PhD (Time – 25%) ................. $2473.00
2. Materials, Reagents and Supplies................................................ $3000.00
3. Analytical Service ........................................................................ $314.00
4. Indirect Expenses......................................................................... $463.00
Total Second Quarter Research Budget .................................................. $6250.00
SAMPLE
TITLE:
FUNDING AGENCIES: Goldman Philanthropic Partnerships
FUNDING PERIOD:
RESEARCH PLAN BUDGET
A. Budget for Research Plan, 3rd quarter:
October 1, 2003-December 31, 2003
1. Salary and Fringe Benefits
Principal Investigators: Ilya Koltover, PhD................................. $0.00
Project Investigator: Jianqin Liu, PhD (Time – 25%) ................. $2473.00
2. Materials, Reagents and Supplies................................................ $3000.00
3. Analytical Service ........................................................................ $314.00
4. Indirect Expenses......................................................................... $463.00
Total Third Quarter Research Budget ..................................................... $6250.00
TITLE:
FUNDING AGENCIES: Goldman Philanthropic Partnerships
FUNDING PERIOD:
RESEARCH PLAN BUDGET
A. Budget for Research Plan, 4th quarter:
January 1, 2004-March 31, 2004
1. Salary and Fringe Benefits
Principal Investigators: Ilya Koltover, PhD................................. $0.00
Project Investigator: Jianqin Liu, PhD (Time – 25%) ................. $2473.00
2. Materials, Reagents and Supplies................................................ $3000.00
3. Analytical Service ........................................................................ $314.00
4. Indirect Expenses......................................................................... $463.00
Total Fourth Quarter Research Budget................................................... $6250.00
SAMPLE
TITLE:
FUNDING AGENCIES: Goldman Philanthropic Partnerships
FUNDING PERIOD:
RESEARCH PLAN BUDGET
A. Budget for Research Plan, Overall year 1: April 1, 2003-March 31, 2004
1. Salary and Fringe Benefits
Principal Investigators: Ilya Koltover, PhD................................. $0.00
Project Investigator: Jianqin Liu, PhD (Time – 25%) ................. $9892.00
2. Materials, Reagents and Supplies................................................ $12000.00
3. Analytical Service ........................................................................ $1256.00
4. Indirect Expenses......................................................................... $1852.00
Total Research Budget............................................................................. $25000.00
SAMPLE
Research Project Reporting Template
TITLE: Development of a Bio-mimetic Drug Delivery System
FUNDING AGENCIES: Goldman Philanthropic Partnerships, Rockefeller Brothers
Fund
GPP PROJECT CODE #: 03-0331IK
FUNDING PERIOD: July 1, 2003-September 30-2003
PRINCIPAL INVESTIGATOR: Dr. Ilya Koltover
SECOND QUARTER AIMS AND OBJECTIVES OF RESEARCH PLAN
A. Research Objective 3: Preparation and characterization of liposomes
1. Description of research work completed:
After completion of the first quarter research, we have had a few remaining problems
with lipid/peptide synthesis and purification. In particular, purification of the proposed
fully biosynthetic functional molecules P1 and P2 has proven to be difficult, while semi-
synthetic approach, coupling terminal peptides to novel PEG-derivatised lipid anchor
molecules appeared to be promising. Over the last two months, our research has lead to
the following conclusions, that mainly finalize the decisions on the synthetic strategy:
I. Fully biosynthetic approach is feasible (by incorporating the P1 and P2 into fusion
proteins and facilitating purification), but vary time consuming. The full P1 and P2
consisting of three polypeptide segments (hydrophobic anchor, flexible linker and
targeting/pH trigger domain), are difficult to characterize (e.g. by MS or NMR) and hard
to handle (solubility). Given the limited man-power and budget of the project, we will
abandon (for the time being) this approach for the semi-synthetic strategy.
II. Three semi-synthetic lipids has been made, incorporating pH sensitive peptide
prepared by SPPS technique. The three molecules have three different length of linker
between hydrophobic (hydrocarbon) portion of the lipid and the functional peptide: no
linker, short (MW 600) linker, and long (MW 3,000) linker. Study of the relation
between linker length and lipid functionality was one of the central goals of the proposed
research, and this synthetic accomplishment is an important milestone in the project. The
synthesis is novel and publishable.
The synthetic lipid/pH peptide molecules were incorporated into liposomes (DOPC lipid
as the main component) at 0.1, 0.5, and 1 mol% concentrations. This was accomplished
from dry preparations of the lipid mixtures by hydration and extrusion. An ethanol
injections procedure was also adopted for the preparation of somewhat larger (10µm vs
0.2µm) size liposomes that will be useful for drug release tests.
The liposomes are characterized by light scattering and negative staining TEM. So far, it
appears that incorporation of the functional lipids into liposomes does not destabilize
them at neutral pH.
2. What problems were encountered?
See above.
3. What discoveries were made?
See above
4. How did results of work on this objective create any need to alter the plan going
forward?
See above. The project is focusing now on semi-synthetic lipids. This is a natural
convergence based on research progress to date.
B. Research Objective 4: Incorporation of molecules into liposomes
1. Description of research work completed:
Model compounds calceine and fluorescent dextran (MW6,000) were encapsulated inside
liposomes with pH-sensitive lipid membranes both during extrusion and ethanol
injection. Un-encapsulated material was separated either by centrifugation or gel
filtration. Both procedures appear to be successful. Efficiency of encapsulation (e.g.
loading ratios) are being evaluated, and we should be able to focus on a single procedure
in the next couple of weeks.
2. What problems were encountered?
This stage of the project appears to be less problematic, compare with the synthesis of
lipids. pH-triggered release studies will be started as soon as this report is approved.
3. What discoveries were made?
4. How did results of work on this objective create any need to alter the plan going
forward?
C. What new hiring or equipment purchases were made during this period?
Dr. Michael Zhuravel (postdoc) has taken over the project (from the departed Jianqin
Liu). His salary was not, thus far, charged to the grant, but will be in the next three
months.
D. What results were published through what vehicles, internal and external? What
other publications did the researchers publish that would be of interest to those
supporting your research, or what other information was published that might be
relevant to this research? (please include citations only)
E. Is there anything additional you would like to share with those supporting your
research?
